Title:

Wykrywanie pryszczycy z zastosowaniem PCR

Subtitle:

Wykrywanie pryszczycy z zastosowaniem PCR

Creator:

Paprocka, Grażyna ; Kęsy, Andrzej ; Fitzner, Andrzej ; Niedbalski, Wiesław ; Płucienniczak, Grażyna

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

1997

Subject and Keywords:

biotechnology

Abstract:

The polymerase chain reaction (PCR) method has been applied to the detection of FMD viralRNA in samples taken during the clinical stage of FMD from calf. Total RNA was extracted withguanidinum thiocyanate-phenol-chloroform method and reversely transcribed using AMV-reversetranscriptase. cDNA was used as a template for the amplification by PCR of the 672 bp of theVPl coding sequence. The amplified fragment of cDNA was cloned in the phagemid pBS(+). Thefirst DNA strand was sequenced and consistently an amino acid sequence was established.Comparison between VPl amino acid sequence of FMDV types C and earlier described type Owas performed.

Relation:

Biotechnologia, vol.39, 4 (1997)-.

Volume:

39

Issue:

4

Start page:

55

End page:

61

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-35

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open