Title:

Purification and some characteristics of Penicillium citrinum lipase

Subtitle:

Purification and some characteristics of Penicillium citrinum lipase

Creator:

Maliszewska, Irena H.

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

1997

Type of object:

Journal/Article

Subject and Keywords:

biotechnology

Abstract:

An extracellular lipase (glycerol ester hydrolases E.C. 3.1.1.3.) was isolated from a culturefiltrate of Penicillium citńnum. The purification procedure included ammonium sulfate precipitation, ultrafiltration and chromatography on Octyl-Sepharose CL-4B. The enzyme was 400-foldpurified with 9.66% yield. The molecular weight has been estimated by polyacrylamide gel electrophoresis under denaturing conditions at 26 000. On the other hand, lipase forms active dimersand tetramers aggregates as observed after native PAGE. Lipase from Penicillium citrinum showeda preference for triacylglycerols. It is non-specific and hydrolyzes each of the three ester bondsof triacylglycerols. The enzyme showed a maximum activity at pH 7.2 at 30°C and was stablein the range of pH 6.0-7.5 and the temperature of 10°C - 40°C.

Relation:

Biotechnologia, vol.38, 3 (1997)-.

Volume:

38

Issue:

3

Start page:

109

End page:

120

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open