Title:

Ekspresja genów heterologicznych w Escherichia coli na modelowo ujętym przykładzie interferonu gamma

Subtitle:

Ekspresja genów heterologicznych w Escherichia coli na modelowo ujętym przykładzie interferonu gamma

Creator:

Cieślikowski, Tomasz

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

1997

Type of object:

Journal/Article

Subject and Keywords:

biotechnology

Abstract:

Interferon gamma is a molecule which may serve as a good object for studying and explainingthe role of expression limiting conditions.Three levels of limiting factors modifications, i. e. operon choice and structure, changes inthe secondary mRNA structure, and the influence of post-translational modulation processes aredescribed. The relationship between the operon stucture and expression level for other heterologous proteins is compared with that of IFN-gamma. In particular, the shape of ribosome bindingsite (RBS) region and its affected different nucleotide substitutions are presented.The rule of N-terminal and C-terminal end changes for protein stability and inclusion bodyformation is also shown.

Relation:

Biotechnologia, vol.37, 2 (1997)-.

Volume:

37

Issue:

2

Start page:

71

End page:

92

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open