Title:

Anti-HIV Activities and Mechanisms of Antisense Oligonucleotides

Subtitle:

Anti-HIV Activities and Mechanisms of Antisense Oligonucleotides

Creator:

Hatta, Toshifumi ; Inagawa, Takabumi ; Kuwasaki, Tomoyuki ; Kinzuka, Yasuhiro ; Takai, Kazuyuki ; Yokoyama, Shigeyuki ; Nakashima, Hideki ; Yamamoto, Naoki ; Takaku, Hiroshi

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

1996

Description:

Polish - Japan Issue

Subject and Keywords:

biotechnology

Abstract:

We demonstrated that unmodified and modified (phosphorothioate) oligonucleotides preventcDNA synthesis by the AMV, MMLV, and HIV reverse transcriptases. Antisense oligonucleotide/RNA hybrids specifically arrest primer extension. The blockage involves the degradation ofthe RNA fragment bound to the antisense oligonucleotide by the reverse transcriptase associatedRNase H activity. However, the phosphorothioate oligomer inhibited polymerization by bindingto the AMV and MMLV RTs, rather than to the template RNA, whereas there was no competitivebinding of the phosphorothioate oligomer on the HIV RT during reverse transcription. Observation{of FlTC-S-ODN-reu-treated MOLT-4 cells with a confocal laser scanning microscope, revealedi diffuse fluorescence, apparently within the cytoplasm. Interestingly, fluorescent signals were; accumulated in the nuclear region of chronically infected MOLT-4/H1V-1 after a 60 min incubation. We also describe the long-term treatment of human immunodeficiency virus-infectedcells with antisense phosphorothioate oligonucleotides.

Relation:

Biotechnologia, vol.35, 4 (1996)-.

Volume:

35

Issue:

4

Start page:

116

End page:

131

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-31

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

eng

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open