Title:

Interaction of Antisense Oligonucleotides with DNA and Proteins Studied by Fluorescence Polarization Spectroscopy

Subtitle:

Interaction of Antisense Oligonucleotides with DNA and Proteins Studied by Fluorescence Polarization Spectroscopy

Creator:

Murakami, Akira ; Nakaura, Misuzu ; Uematsu, Hiroki ; Fujimura, Naoko ; Iwase, Reiko ; Yamaoka, Tetsuji

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

1996

Description:

Polish - Japan Issue

Subject and Keywords:

biotechnology

Abstract:

In order to evaluate the antisense effects in biological systems, the interaction of antisensemolecules with biological substances such as DNAs and human serum proteins was studied byfluorescence depolarization spectroscopy. When an antisense oligonucleotide anchoring fluorescein (F-AS) was mixed with DNAs or proteins, the anisotropy value changed, indicating thatantisense oligonucleotides appreciably interact with DNAs and serum proteins, and that suchinteraction may play important roles on antisense gene regulation.

Relation:

Biotechnologia, vol.35, 4 (1996)-.

Volume:

35

Issue:

4

Start page:

153

End page:

166

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-31

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

eng

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open