Tytuł:

Antisense Oligonucleotides Against Inducible Nitric Oxide Synthase Reduce NO Production in RAW 264.7 Cells

Inny tytuł:

Antisense Oligonucleotides Against Inducible Nitric Oxide Synthase Reduce NO Production in RAW 264.7 Cells

Twórca:

Bilecki, Wiktor ; Okruszek, Andrzej ; Kwinkowski, Marek ; Sanak, Marek ; Przewłocki, Ryszard

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Data wydania/powstania:

1996

Opis:

Polish - Japan Issue

Temat i słowa kluczowe:

biotechnology

Abstrakt:

Several oligodeoxyribonucleotides in a nuclease-resistant phosphorothioate form (S-ODNs)targeted to iNOS mRNA were tested in RAW 264.7 cells as potential antisense inhibitors.Antisense S-ODNs inhibited iNOS activity in a time- and dose-dependent manner. Maximalinhibitory effect was >90% while control S-ODNs were ineffective. Application of Lipofectin Reagent, which itself had no significant effect, greatly increased antisense activity. Decreased levelsof the target mRNA, as demonstrated by reverse transcriptase-polymerase chain reaction (RTPCR), suggest the RNase H mediated mechanism.

Czasopismo/Seria/cykl:

Biotechnologia, vol.35, 4 (1996)-.

Tom:

35

Zeszyt:

4

Strona pocz.:

173

Strona końc.:

182

Typ zasobu:

Text

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; IChB B-31

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

eng

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open