Title:

Factors influencing expression of recombinant genes in vitro in E. coli. Part II. Culture conditions In bioreactor

Subtitle:

Factors influencing expression of recombinant genes in vitro in E. coli. Part II. Culture conditions In bioreactor

Creator:

Grajek, Włodzimierz

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

1995

Subject and Keywords:

biotechnology

Abstract:

High expression yield and high volumetric productivity of fermentations employing recombinant E. coli need special processing. For recombinat protein production two strategies can beapplied. One is to carry out one step fermentation with simultaneous gene expression andelimination of vector-less segregants. The other is to carry out two step fermentation, in whichthe first step is the cell biomass production without gene expression followed by the inductionof gene expression in the second step. In this review different methods of controlling of recombinant gene expression, such as antibiotic pressure, specific mutant application, immobilizedcell bioreactors, selective cell recyrculation, chemical regulations, the use of temperature andoxygen sensitive promoters are presented.

Relation:

Biotechnologia, vol.28, 1 (1995)-.

Volume:

28

Issue:

1

Start page:

103

End page:

113

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-24

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open