Subtitle:

Thermodynamic aspects of oligonucleotide duplex formation and their effect on selectivity of hybridization in antisense strategy

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

The methods of evaluation of thermodynamic parameters for oligonucleotide order-disordertransitions are presented. These parameters are useful for the prediction of stability of duplexesformed by these oligonucleotides with RNA matrix. The common opinion, that better antisenseproperties can be achieved by the increase of oligonucleotide length is critically evaluated. Theproblem of optimal length of oligonucleotide for antisense purposes is discussed by taking intoaccount the selectivity of hybridization.

Relation:

Biotechnologia, vol.27, 4 (1994)-.

Volume:

27

Issue:

4

Start page:

59

End page:

68

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:148132 ; IChB B-23

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open