Subtitle:

Crystallization of nucleic acids

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Crystallization is the most important and challenging step in the determination of the structure of biomolecules and macromolecules by diffraction methods.Macromolecules are extremely complex physical-chemical system whose properties vary as afunction of many environmental influences such as temperature, pH, ionic strength and solventcomposition. Therefore, a number of factors influencing macromolecule crystal growth ought tobe considered. Despite of considerable interest in the approaches allowing for continuous monitoring and setting of crystal growth conditions, in practice, numerous trials ought to be performedin order to obtain appropriate crystals characterized by their optimal geometrical parameters,crystallographic symmetry, unit cell and, most importantly, their high resolution.The vapour diffusion and the microdialysis methods are discussed as the most widely usedcrystallization methods of macromolecules.

Relation:

Biotechnologia, vol.23, 4 (1993)-.

Volume:

23

Issue:

4

Start page:

137

End page:

146

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:149099 ; IChB B-19

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open