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Klonowanie genów metodą hybrydyzacji różnicowej
Subtitle:Gene cloning using subtractive hybridization
Creator:Rusin, Marek ; Szala, Stanisław
Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:Genes expressed in temporally, environmentally or tissue specific way can be cloned using subtractivehybridization. It involves hybridization of single stranded cDNA from one cell type with mollar excess ofpolyA+RNAfrom another cell type. Unhybridized, cell specific cDNA is subsequently used or as subtractiveprobe or as material for construction of subtractive cDNA library. Modifications of this basic strategy arealso discussed.
Relation:Biotechnologia, vol.17, 2 (1992)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
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