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Application of molecular biology methods for blood testing
Subtitle:Application of molecular biology methods for blood testing
Creator: Publisher:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Subject and Keywords: Abstract:In this paper a simple, rapid and reliable test for sex diagnosis has been described. The test is basedon detection of alphoid satellite sequences in undigested genomic DNA using the polymerase chain reaction (PCR) and dried blood specimens serving as the source of DNA. When female DNA was used as atemplate for the reaction, only the expected X-chromosome-specific fragment was detected, while withmale DNA both X-specific and Y-specific-fragments were detected. The Y-chromosome-specific fragment was further characterized by restriction enzyme analysis. The Y fragment was detectable when DNAobtained from an equivalent of 5 n\ of spotted blood was used in the reaction. This test may find applications in sexing of human DNA for forensic purposes, in following host versus graft cells in patients withbone marrow transplants from the opposite sex or in searching for XO/XY mosaicism in patients withTurner syndrome.
Relation:Biotechnologia, vol.13-14, 3-4 (1991)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
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