The role of CYP-450 dependent arachidonic acid (AA) metabolites (vasoconstrictor 20-HETE and vasodilator EETs) and NO in control of blood pressure (MABP) and kidney function remains unclear. NO affects the activity of heme-containing enzymes, like CYP-450 related monooxygenases, moreover, their activity depends on Na(+) intake. The focus of this review and underlying studies is on the role of high sodium intake (pro-hypertensive factor) in interrelation between CYP-450 and NOS. The acute vs. chronic non-selective inhibition of CYP-450 AA metabolites (ABT), and selective inhibition of 20-HETE (HET 0016) has also been tested. The renal artery flow (RBF, Transonic probe), medullary blood flow (MBF, laser-Doppler flux), renal excretion, and medullary tissue NO (selective electrode) were measured in male anaesthetized Wistar rats. We conclude that on standard Na(+) intake, opposed effects of 20-HETE and EETs are almost in equilibrium; however, in the renal circulation the vasodilator EETs influence slightly prevails. High sodium intake stimulates NOS, which limits CYP-450 impact on MABP and kidney function. However, this protection disappears after prolonged sodium intake. Long-lasting high sodium intake lowers NO bioavailability and promotes systemic and intrarenal vasoconstrictor activity of 20-HETE. Opposed effects of NO and AA metabolites of CYP-450 on water and solute excretion are also described.
Kuczeriszka, Marta Badzyńska, Bożena Kompanowska-Jezierska Elżbieta
Sporkova, Alexandra Jichova, Sarka Huskova, Zuzanna Koopkan, Libor Nishiyama, Akira Hwang, Sung H. Hammock, Bruce D Imig, John D Kompanowska-Jezierska, Elżbieta Sadowski, Janusz Kramer, Herbert J Cervenka, Ludek
Walkowska, Agnieszka Badzyńska, Bożena Kompanowska-Jezierska, Elżbieta Johns, EJ Sadowski, Janusz
Kuczeriszka , Marta Dobrowolski, Leszek Walkowska, Agnieszka Sadowski, Janusz Kompanowska-Jezierska, Elżbieta
Walkowska, Agnieszka Kuczeriszka , Marta Sadowski, Janusz Olszyński, Krzysztof Hubert Dobrowolski, Leszek Cervenka, Ludek Hammock, BD Kompanowska-Jezierska, Elżbieta