Object

Title: Cytochrome P-450 monooxygenases in control of renal haemodynamics and arterial pressure in anaesthetized rats

Contributor:

Laboratory of Renal and Body Fluid Physiology, M. Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

Publisher:

Polish Physiological Society

Place of publishing:

Krakow

Abstract:

The renal regulatory role of cytochrome P450 dependent metabolites of arachidonic acid (AA), vasodilator epoxyeicosatrienoic acids (EETs) and vasoconstrictor 20-hydroxyeicosatetraenoic acid (20-HETE), was examined in anaesthetised rats. We measured renal artery flow (RBF), cortical (CBF) and medullary (MBF) perfusion (laser-Doppler) and medullary tissue nitric oxide (NO, selective electrode), after non-selective inhibition of CYP-450 pathway with 1-aminobenzotriazole (ABT, 10 mg/kg i.v.) or after selective inhibition of 20-HETE synthesis with HET0016 (Taisho Co, Yoshino-cho, Japan), infused into renal artery at 0.3 mg/kg/h or into renal medulla at rates increasing from 0.15 to 1.5 mg/kg/h. ABT caused significant (by 13.7%) decrease in RBF without changing MBF. Renal arterial HET0016 increased MBF (not RBF or CBF) from 152+/-12 to 174+/-12 perfusion units (+16%, P<0.001), while medullary tissue nitric oxide was significantly increased (P<0.001). After renal medullary HET0016, renal perfusion indices were significantly higher than after HET0016 solvent (beta-cyclodextrin). Total renal blood flow seems to be under vasodilator control of EETs whereas renal medullary perfusion under tonic suppression by 20-HETE. The data document, for the first in the whole kidney studies, the functional antagonism of 20-HETE and NO.

Relation:

Journal of Physiology and Pharmacology

Volume:

57

Issue:

suppl.11

Start page:

179

End page:

185

Format:

text/xml

Resource Identifier:

oai:rcin.org.pl:57189 ; oai:rcin.org.pl:57189

Language:

eng

Language of abstract:

eng

Rights:

Creative Commons Attribution BY 3.0 PL license

Terms of use:

Copyright-protected material. [CC BY-NC 3.0 PL] May be used within the scope specified in Creative Commons Attribution BY-NC 3.0 PL license, full text available at:

Digitizing institution:

Mossakowski Medical Research Center PAS

Original in:

Library of the Mossakowski Medical Research Center PAS

Projects co-financed by:

Programme Innovative Economy, 2010-2014, Priority Axis 2. R&D infrastructure

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