Title:

Sekwencjonowanie największego chromosomu Paramecium tetraurelia - przykład opracowania metody

Subtitle:

Journal of Biotechnology, Computational Biology and Bionanotechnology

Creator:

Nowak, Jacek K.

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Date issued/created:

2010

Description:

prace przeglądowe

Subject and Keywords:

biotechnologia

Abstract:

The largest, megabase, somatic chromosome of P. tetraurelia was isolated and sequenced in order to explore its organization and gene content. The AT-rich chromosome is compact with very small introns, short intergenic regions and a coding density of at least 74%, higher than that reported for budding yeast or any other free-living eukaryote. Homology to known proteins could be detected only for 57% of the 464 potential protein coding genes. Subsequently, the megabase chromosome sequence was used during the whole genome sequencing project as a reference to evaluate sequence assembly and gene annotation accuracy. In a pilot project of the global analysis of P. tetraurelia gene expression during autogamy, DNA microarrays were used. Statistical data analysis allowed the identification of four clusters of co-ex- pressed genes. Screening for silencing phenotypes of 15 autogamy specific genes revealed that 4 genes were essential during vegetative growth and 3 others were essential for successful sexual process.

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Relation:

Biotechnologia, vol.91, 4 (2010)-.

Volume:

91

Issue:

4

Start page:

76

End page:

90

Resource type:

Tekst

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

IChB B-86

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

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