Title:

The activity of oxidative enzymes in neuroglia cultured in vitro. II. Dehydrogenases linked with ectenzyme I and succinate dehydrogenase

Creator:

Kraśnicka, Zuzanna ; Mossakowski, Mirosław Jan (1929–2001)

Publisher:

Państwowy Zakład Wydawnictw Lekarskich

Place of publishing:

Warszawa

Date issued/created:

1966

Type of object:

Czasopismo/Artykuł

Subject and Keywords:

oxidative enzymes ; neuroglia

Abstract:

Histochemical studies were carries out with tissue cultures of glia from newborn rats. Cultures aged 1, 2, 3, 4, 5, 6, 7, 10, 11, 13, 14—19, 21, 25, 28 and 30 days were studied. Activity of the following enzymes was investigated: dehydrogenase of coenzyme I, succinic, lactic, glutamic, malic and ethyl alcohol dehydrogenases.Activity of all the studied enzymes, with the exception of the dehydrogenase of coenzyme I, was lower in the early stages of growth (1—10 days) than in later stages. Tetrazolium reductase, which exhibited the highest activity, was not affected by the age of the culture.Significant differences in the distribution of enzymatic activity were found in relation to the type of glial cells and age of the culture.The dehydrogenase of coenzyme 1 and lactic dehydrogenase showed the highest activity. Glutamic, succinic and malic dehydrogenases showed lower activity, and alcohol dehydrogenase the lowest activity, which increased in older cultures.Marked individual differences in intensity of enzymatic activity of different cells of the same type were noted, presumably in connection with the functional state of the cells.The intensity of enzymatic activity in glial cell in vitro is similar to that in reactive glia in vivo.

Relation:

Polish Medical Journal

Volume:

5

Issue:

6

Start page:

1385

End page:

1404

Resource type:

Tekst

Detailed Resource Type:

Artykuł

Format:

pdf

Language:

eng

Language of abstract:

eng

Rights:

Licencja Creative Commons Uznanie autorstwa 4.0

Terms of use:

Zasób chroniony prawem autorskim. [CC BY 4.0 Międzynarodowe] Korzystanie dozwolone zgodnie z licencją Creative Commons Uznanie autorstwa 4.0, której pełne postanowienia dostępne są pod adresem: ; -

Digitizing institution:

Instytut Medycyny Doświadczalnej i Klinicznej im. M. Mossakowskiego Polskiej Akademii Nauk

Original in:

Biblioteka Instytutu Medycyny Doświadczalnej i Klinicznej im. M. Mossakowskiego PAN

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

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