Object

Title: The activity of oxidative enzymes in neuroglia cultured in vitro. II. Dehydrogenases linked with ectenzyme I and succinate dehydrogenase

Publisher:

Państwowy Zakład Wydawnictw Lekarskich

Place of publishing:

Warszawa

Abstract:

Histochemical studies were carries out with tissue cultures of glia from newborn rats. Cultures aged 1, 2, 3, 4, 5, 6, 7, 10, 11, 13, 14—19, 21, 25, 28 and 30 days were studied. Activity of the following enzymes was investigated: dehydrogenase of coenzyme I, succinic, lactic, glutamic, malic and ethyl alcohol dehydrogenases.Activity of all the studied enzymes, with the exception of the dehydrogenase of coenzyme I, was lower in the early stages of growth (1—10 days) than in later stages. Tetrazolium reductase, which exhibited the highest activity, was not affected by the age of the culture.Significant differences in the distribution of enzymatic activity were found in relation to the type of glial cells and age of the culture.The dehydrogenase of coenzyme 1 and lactic dehydrogenase showed the highest activity. Glutamic, succinic and malic dehydrogenases showed lower activity, and alcohol dehydrogenase the lowest activity, which increased in older cultures.Marked individual differences in intensity of enzymatic activity of different cells of the same type were noted, presumably in connection with the functional state of the cells.The intensity of enzymatic activity in glial cell in vitro is similar to that in reactive glia in vivo.

Relation:

Polish Medical Journal

Volume:

5

Issue:

6

Start page:

1385

End page:

1404

Detailed Resource Type:

Article

Format:

pdf

Resource Identifier:

oai:rcin.org.pl:70603

Language:

eng

Language of abstract:

eng

Rights:

Creative Commons Attribution BY 4.0 license

Terms of use:

Copyright-protected material. [CC BY 4.0] May be used within the scope specified in Creative Commons Attribution BY 4.0 license, full text available at: ; -

Digitizing institution:

Mossakowski Medical Research Center PAS

Original in:

Library of the Mossakowski Medical Research Center PAS

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open

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