Object

Title: Resistance of transgenic tobacco lines to Potato Virus Y

Creator:

Czubacka, Anna ; Doroszewska, Teresa

Date issued/created:

2010

Resource Type:

Article

Contributor:

Institute of Bioorganic Chemistry PAS ; Committee on Biotechnology PAS

Abstract:

Potato virus Y (PVY) causes a disease which reduces the quality of raw material as well as the yield. This study on resistance included tobacco transgenic lines belonging to four cultivars modified with three genetic constructions, containing Lettuce mosaic virus coat protein gene (LMV CP) and PVY replicase gene used in sense (ROKY1) and antisense (ROKY2) orientation. The plants were inoculated with PVY isolates under greenhouse conditions and tested by DAS-ELISA. The highest effectiveness of antiviral protection was noticed in case of the lines with transgene LMV CP, lower for ROKY2, and the lowest for ROKY1. In following generations, the percentage increase of a number of plants resistant to PVY was observed within the lines containing transgene LMV CP. Cultivar MN 944 showed to be the best genetic background for that transgene, while cultivar AC Gayed for transgene ROKY.

Relation:

Biotechnologia, vol.89, 2 (2010)-.

Volume:

89

Issue:

2

Start page:

72

End page:

82

Format:

application/pdf

Resource Identifier:

oai:rcin.org.pl:71582 ; IChB B-84

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Rights:

Creative Commons Attribution BY 4.0 license

Terms of use:

Copyright-protected material. [CC BY 4.0] May be used within the scope specified in Creative Commons Attribution BY 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Object collections:

Last modified:

Feb 18, 2020

In our library since:

May 27, 2019

Number of object content hits:

43

All available object's versions:

https://rcin.org.pl/publication/93827

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