Title:

Somaclonal variation in micropropagated Hemerocallis sp. plants determined by phenotype and molecular markers, RAPD and ISSR

Subtitle:

Somaclonal variation in micropropagated Hemerocallis sp. plants determined by phenotype and molecular markers, RAPD and ISSR

Creator:

Podwyszyńska. Małgorzata ; Gabryszewska, Eleonora ; Korbin, Małgorzata ; Jasiński, Artur

Contributor:

Institute of Bioorganic Chemistry PAS ; Committee on Biotechnology PAS

Publisher:

Instytut Chemii Bioorganicznej PAN ; Komitet Biotechnologii PAN

Date issued/created:

2010

Subject and Keywords:

biotechnology

Abstract:

The genetic fidelity of the micropropagated daylily plants was evaluated by a phenotypic observation and with the molecular markers, randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) techniques. The plants of nine daylily cultivars propagated in vitro for the period of one to five years and grown outdoors in the years 2002 to 2007 were used in the study. The somaclonal variants occurred within the micropropagated plants of the five genotypes. The somaclones differed from the true-to-type plants in terms of colour and shape of the flowers. Depending on the genotype, the frequency of somaclonal variation (SV) ranged from 1.4% to 100%. The RAPD and ISSR analyses of the selected somaclones confirmed the polymorphism between true-to-type plants and somaclones on the level of DNA.

Relation:

Biotechnologia, vol.89, 2 (2010)-.

Volume:

89

Issue:

2

Start page:

114

End page:

124

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

IChB B-84

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open