The aim of the presented research was to examine the morphogenetic response of Polemonium coeruleum explants. The donor material were 10-day-old seedlings. Surface sterilized seeds were germinated on MS medium supplemented with CA3 (1 mg dnr^). Seedling explants (shoot tips, fragments of cotyledons, hipocotyls and roots) were isolated and transferred onto solidified MS medium supplemented with different types of cytokinins (BA, KN, ZEA, 2iP) at concentrations 1.0, 3.0 and 5.0 mg dm ^ in combination with NAA (0.1 mg dnr^). All explant types were characterized by callus proliferation. It was observed that calli developed on the entire surface of hipocotyl and root fragments. On the other hand, shoot tips and cotyledonary petioles formed callus tissue at the cut ends, and petioles only at abaxial ends. The growth of calli on all explant types was strongly stimulated by ZEA. Among the explants tested, only shoot tips exhibited shoot organogenesis. The highest frequency of shoot organogenesis was observed when the explants were cultured on a medium supplemented with 5.0 mg dm-3 BA (lOO^o) or 5.0 mg dnr^ ZEA (97%). The highest shoot number per explant was obtained in the presence of 5.0 mg dnr^ZEA (8.4 on average). The presence of BA or ZEA in the proliferation medium inhibited rhizogenesis and the elongation growth of shoots. However, root organogenesis was supported by KN added into the medium.
Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.
Feb 18, 2020
May 27, 2019
|Wpływ cytokinin na odpowiedź morfogenetyczną eksplantatów pochodzących z siewek Polemonium coeruleum||Feb 18, 2020|
Trejgell, Alina Tretyn, Andrzej
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Studzińska, Anna Tyburski, Jarosław Daca, Patrycja Tretyn, Andrzej
Deja-Sikora, Edyta Sikora, Marcin Gołębiewski, Marcin Tretyn, Andrzej
Tyburski, Jarosław Studzińska, Anna Daca, Patrycja Tretyn, Andrzej
Nowak, Jacek K.
Institute of Bioorganic Chemistry PAS Committee on Biotechnology PAS