Embryogenic callus of Leucojum aestivum was transferred to solid and liquid media in bioreactor RITA® vessels. Callus growth on the liquid medium enriched with 2 and 5 qM Picloram and 0.5 qM BA was characterized by a higher multiplication index. The greatest number of somatic embryos was observed on the callus multiplied in bioreactor using 5-minute flushing with the medium. Globular embryos developed into torpedo-stage embryos and plantlets under the influence of NAA (0.5 qM) and zeatin (5 qM). Liquid medium promoted the development of L aestivum somatic embryos and plantlets.
Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.
Feb 18, 2020
May 27, 2019
|Mikrorozmnażanie Leucolum aesHvum L. w bioreaktorze RITA®||Feb 18, 2020|
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