Title:

Rooting in vitro and acclimatization in the greenhouse of herbaceous peony plantlets

Subtitle:

Rooting in vitro and acclimatization in the greenhouse of herbaceous peony plantlets

Creator:

Kawa- Miszczak, Ludwika ; Gabryszewska, Eleonora

Contributor:

Institute of Bioorganic Chemistry PAS ; Committee on Biotechnology PAS

Publisher:

Instytut Chemii Bioorganicznej PAN ; Komitet Biotechnologii PAN

Date issued/created:

2010

Subject and Keywords:

biotechnology

Abstract:

The influence of sucrose concentration (10 and 30 g 1'). low level of nitrogen salts (1/8 KNO3, 1/8 NH3NO4), auxins (IBA 1 mg 1' + lAA 1 mg 1' + N/\A 0,01 mg 1'), and temperature (15°C, 20°C) on the rooting in vitro and acclimatization in the greenhouse of two cultivars (‘Jadwiga’, ‘Professor Wójcicki’) of Paeonia lactiflora were investigated. The level of endogenous carbohydrates in the peony shoots and roots during the rooting and acclimatization phases were analysed. There were a higher rooting and acclimatization percentage of ‘Professor Wójcicki’ cultivar than ‘Jadwiga’ cultivar. Also, more roots were produced by shoots of‘Professor Wójcicki’ cultivar. The presence of auxins in the medium and the higher concentration of sucrose (30 g I ') stimulated number of roots/shoot. On the other hand, the higher rooting percentage was found on the auxin-free medium in the presence of high level of sucrose. The shoots of ‘Professor Wójcicki’ rooted best when cultured at 20°C, but the shoots of ‘Jadwiga’ at lower temperature - 15°C. During the rooting stage, the major sugars detected in the peony microplants were sucrose, glucose and fructose. After four weeks of acclimatization, the plantlets accumulated starch and showed strong inhibition of shoot growth, and dormant buds were developed.

Relation:

Biotechnologia, vol.89, 2 (2010)-.

Volume:

89

Issue:

2

Start page:

172

End page:

179

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

IChB B-84

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open