Title:

Optimization of simultaneous saccharification and fermentation of the native starch by using response surface method

Subtitle:

Optimization of simultaneous saccharification and fermentation of the native starch by using response surface method

Creator:

Białas, Wojciech ; Wojciechowska, Dorota ; Szymanowska, Daria ; Grajek, Włodzimierz

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2009

Description:

prace eksperymentalne

Subject and Keywords:

biotechnology

Abstract:

Ethanol production, by a simultaneous saccharification and fermentation process (SSF) of native starch from corn flour, has been performed by Saccharomyces cerevisiae (Ethanol Red) and granular starch hydrolyzing enzyme (STARGEN 001). The quantitative effects of mash concentration, enzyme dose and pH were investigated by the use a Box-Wilson central composite design protocol. It was found that for native corn starch, maximum ethanol concentration of 110,36 g/1 was obtained using a mash concentration of 25%, which resulted in ethanol yield of 85,71%. The optimum conditions for the above yield were found for the enzyme dose of 2,05 ml/kg and pH of 5.0. These results indicated that by using the central composite design, it is possible to determine accurate values of the fermentation parameters for maximum ethanol production.

Relation:

Biotechnologia, vol.87, 4 (2009)-.

Volume:

87

Issue:

4

Start page:

183

End page:

199

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-82

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol ; pol

Language of abstract:

eng

Temporal coverage:

2009

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open