Title:

The influence of DNA isolation’s method on result of genetic similarity analysis made with RAPD technique

Subtitle:

The influence of DNA isolation’s method on result of genetic similarity analysis made with RAPD technique

Creator:

Dobrzycka, Agnieszka ; Broda, Zbigniew

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2009

Subject and Keywords:

biotechnology

Abstract:

The study purpose was to examine the influence of DNA isolation’s method on result of genetic similarity analysis made with RAPD technique. Two methods of DNA isolation were used - the Tomson-Henry method, which provides low purified DNA, and the column method, which provides high purified DNA. By using DNA isolated with these two methods the RAPD analysis of genetic distance was executed. It was noticed, that using high purified DNA resulted in higher number of amplification’s products, and consequently in larger accuracy of analysis.

Relation:

Biotechnologia, vol.86, 3 (2009)-.

Volume:

86

Issue:

3

Start page:

182

End page:

191

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-81

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

2009

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open