Subtitle:

Structural basis for the antibacterial activity of the 12-membered-ring mono-sugar macrolide methymycin

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Type of object:

Czasopismo/Artykuł

Abstract:

The crystal structure of the complex of the large ribosomal subunit of the pathogen model Deinococcus radiodurans with the macrolide antibiotic methymycin, bearing a 12 membered macrolactone ring macrolide that contains a single amino sugar, shows that methymycin binds to the peptidyl transferase center (PTC) rather than to the high affinity macrolide binding pocket at the upper end of the ribosomal exit tunnel. This unexpected binding mode results in fairly efficient blockage of the 3’end of the A-site tRNA location, thus indicating the superiority of spatial-functional considerations over the formation of the typical high affinity macrolide interactions that due to the small size of methymycin could have led to incomplete blockage of the exit tunnel. Its binding involves rearrangements of several PTC nucleotides, some of which were shown previously to be flexible. Comparisons between the binding modes of methymycin and other antibiotics are presented and discussed.

Relation:

2009 ; Biotechnologia, vol.84, 1 (2009)-.

Volume:

84

Issue:

1

Start page:

24

End page:

35

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:72725 ; IChB B-79

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

eng

Language of abstract:

eng

Temporal coverage:

2009

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

Otwarty