Title:

Obtaining homozygous carrot plants with the aid of androgenesis in anther cultures

Subtitle:

Obtaining homozygous carrot plants with the aid of androgenesis in anther cultures

Creator:

Górecka, Krystyna ; Krzyżanowska, Dorota ; Kiszczak, Waldemar ; Kowalska, Urszula ; Górecki, Ryszard

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

2008

Subject and Keywords:

biotechnology

Abstract:

Experiments with anther cultures of 22 carrot cultivars were carried out to study the effect of various factors on the effectiveness of embryogenesis in these cultures. The factors included: the stage of microsporogenesis, genotype, training of donor plants and their growth conditions. A modified B5 medium (Gamborg, et al. 1968) containing 500 mg L’ glutamine, 100 mg L ’ serine, 0.1 mg L ' of 2,4D, 0.1 mg L ’ NAA, 100 g L ’ sucrose and 6.5 g L ’ agar were used to induce androgenesis. Regeneration was carried out on MS media and B5 with reduced concentration of sucrose at 20 g L ’ without aminoacids and hormones or with small amount of hormones. Substrates that were a mixture of various components, such as peat, sand, mineral wool and charcoal, were used for adaptation. Ploidy of the obtained plants was determined by cytometry method. Homozygosity of the plants was established using two isoenzymatic systems: PGl — phosphoglucose isomerase, and /\AT — aspartate aminotransferase. Anatomical studies of embryogenesis during antlier cultures were also carried out to confirm the androgenetic origin of embryos.

Relation:

Biotechnologia, vol.81, 2 (2008)-.

Volume:

81

Issue:

2

Start page:

142

End page:

152

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-76

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open