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Wiater, Adrian ; Pleszczyńska, Małgorzata ; Szczodrak, Janusz ; Próchniak, Katarzyna
Publisher:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Description: Subject and Keywords: Abstract:Five different methods described in the literature were used for the isolation of a-(l->3)-glucans from the cell wall of fruiting bodies of Laetiporus sulphureus (Bull.iFr.) Murrill, and their comparative analysis was performed. The separated fungal biopolymers were well-characterized in respect of their structure and some physicochemical properties. Structural analyses, i.e., Fou- rier-transform infra-red (FT-IR) spectroscopy, 'FI nuclear magnetic resonance (NMR) spectroscopy and specific rotation, revealed that the alkali-soluble wall fraction from this basidiomycetous fungus contained about 56% of (1—>3)-linked a-glucans. Four out of five a-(l—>3)-glucans isolated by different methods from the mycelium of the polypore fungus L. sulphureus induced higher activity of fungal and bacterial mutanase than those obtained on mutan. Therefore, the a-(l—>3)-glucans from fruiting bodies ofL sulphureus can be used as a new alternative to streptococcal mutan, which so far has been known as the best inducer of mutanase production.
Relation:Biotechnologia, vol.81, 2 (2008)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
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