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Suitability of Thermus ruber as a source of trehalose synthase
Subtitle:Suitability of Thermus ruber as a source of trehalose synthase
Creator:Sinkiewicz, Izabela ; Synowiecki, Józef
Publisher:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Description: Subject and Keywords: Abstract:Thermus ruber is a producer of trehalose synthase, which catalyses the conversion of maltose into trehalose by intramolecular transglucosylation. The specific activity of cell-free extract of Thermus ruber cultivated on a media without saccharides was 0.016-0.028 U/mg protein and it increased up to 0.086 U/mg in the presence of 0.5?^ maltose in the culture broth. The maximum degree of maltose conversion of about 90“6 was achieved at 10% substrate concentration. The trehalose synthase does not catalyse formation of trehalose from malto- tetraose, maltohexaose and other oligosaccharides. The optimal temperature for enzyme activity was 65°C. A maximum activity of the maltose transglucosylation was performed at pH 6.5. The highest yield of trehalose synthase was attained during cultivation of bacteria at 55°C for 48 h on media composed of 0.5% peptone, 0.1% yeast extract and 0.5% maltose or starch.
Relation:Biotechnologia, vol.80, 1 (2008)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
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