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Konstrukcja plazmidowych wektorów bicistronowych i zastosowanie do transfekcji in vitro
Subtitle:Construction of plasmid bicistronic vectors and their application for in vitro transfection
Creator:Kucharzewska, Paulina ; Zagórska, Anna ; Leja, Justyna ; Jaźwa, Agnieszka ; Gozdecka, Małgorzata ; Józkowicz, Alicja ; Dulak, Józef
Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:Internal ribosome entry site (IRES) sequences, which stand for the basic element of cap 5’-independent translation, are currently widely used to coexpress heterologous genes from one plasmid. In this study construction of four bicistronic plasmids containing IRES and application of these vectors for transfection of in vitro cultured cells were described. The obtained data show that constructed bicistronic plasmids are very efficient in vitro in terms of simultaneous expression of fibroblast growth factor-4 (FGF-4) and vascular endothelial growth factor (VEGF) or one of these factors and green fluorescent protein (GFP) from one plasmid. Interestingly, expression of two genes, although simultaneous, is not equal. It has turned out that IRES-dependent mRNA translation is less efficient than cap 5’-dependent translation of the first gene, which should be taken into account during construction of bicistronic plasmids.
Relation:Biotechnologia, vol.78, 3 (2007)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
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