One of the major hurdles to successful gene therapy is the ability to efficiently introduce a foreign nucleic acid into the tissue of interest. As adenoviruses posses ability to enter rapidly a mammalian host cell and achieve propagation, they are widely used as a tool for delivery transgenes. Recombinant adenoviral vectors of first generation (AdV) contain an expression cassette with exogenous genes and are made replication deficient by the deletion of the E1/E3 region. They offer many advantages for gene delivery: ability to transduce a wide variety of cell types in a cell-cycle independent manner, easy and cheap propagation process to high titers and low pathogenicity for humans. However, AdV also have some disadvantages, namely cytotoxity, immunogenity, transient expression of transgene, which are mostly important in case of clinical trials. Despite those limitations and development of more sophisticated adenoviral systems (helper-dependent adenoviral vectors), AdV of first generation are still widely used for transducting different cell types in vitro, especially those that are refractory to other gene transfer methods, as well as in gene therapy clinical trials.
Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.
Sep 19, 2019
Jul 22, 2019
|Najważniejsze cechy wektorów adenowirusowych||Sep 19, 2019|
Stopa, Magdalena Dulak, Józef Józkowicz, Alicja
Józkowicz, Alicja Dulak, Józef
Rutkowski, Andrzej Jaźwa, Agnieszka Józkowicz, Alicja Dulak, Józef
Kucharzewska, Paulina Zagórska, Anna Leja, Justyna Jaźwa, Agnieszka Gozdecka, Małgorzata Józkowicz, Alicja Dulak, Józef
Gołda, Sławomir Kucharzewska, Paulina Cisowski, Jarosław Florczyk, Urszula Zagórska, Anna Jaźwa, Agnieszka Łoboda, Agnieszka Józkowicz, Alicja Dulak, Józef
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Nowak, Jacek K.