@misc{Rzeski_Wojciech_Propagation_2003, author={Rzeski, Wojciech and Paduch, Roman and Kozdruń, Wojciech and Kaczor, Józef and Czekaj, Hanna and Samorek-Salamonowicz, Elżbieta and Kandefer- Szerszeń, Martyna and Zdzisińska, Barbara and Dawidowicz, Andrzej}, volume={61}, number={2}, copyright={Creative Commons Attribution BY-SA 4.0 license}, journal={Biotechnologia, vol.61, 2 (2003)-.}, howpublished={online}, year={2003}, publisher={Committee on Biotechnology PAS}, publisher={Institute of Bioorganic Chemistry PAS}, language={pol}, abstract={Derzsy desease is a fatal hepatitis ofyoung geese under the age of 5 weeks.The etiological agent is a goose parvovirus (GPV) which is widespread in all major goose farming countries in Europe and Asia. At present, vaccination is theonly method preventing the loss of animals. In vitro GPV replicates only in gooseembryo fibroblasts (GEF) and this way the virus is propagated in order to obtainthe material for diagnostic tests and vaccine production.In this paper, we report the application of bioreactor Celligen Plus for GEFculture and Derzsy disease virus propagation. The method is based on immobilization and culture of cells on porous glass carriers in bioreactor. Comparing tothe traditional cell culture techniques, the described method was over 12-foldmore efficient and allowed to obtain good quality virus pool for Derzsy diseasevaccine preparation.}, title={Propagation of Derzsy disease virus in goose embryo fibroblasts. An application of the Celligen Plus bioreactor}, type={Text}, URL={http://rcin.org.pl/ichb/Content/135753/PDF/POZN271_170595_biotechnologia-2003-no2-rzeski.pdf}, keywords={biotechnology}, }