@misc{Nowak_Jacek_K._Sequencing_2010,
 author={Nowak, Jacek K.},
 volume={91},
 number={4},
 copyright={Creative Commons Attribution BY-SA 4.0 license},
 journal={Biotechnologia, vol.91, 4 (2010)-.},
 howpublished={online},
 year={2010},
 publisher={Committee on Biotechnology PAS},
 publisher={Institute of Bioorganic Chemistry PAS},
 language={pol},
 abstract={The largest, megabase, somatic chromosome of P. tetraurelia was isolated and sequenced in order to explore its organization and gene content. The AT-rich chromosome is compact with very small introns, short intergenic regions and a coding density of at least 74%, higher than that reported for budding yeast or any other free-living eukaryote. Homology to known proteins could be detected only for 57% of the 464 potential protein coding genes. Subsequently, the megabase chromosome sequence was used during the whole genome sequencing project as a reference to evaluate sequence assembly and gene annotation accuracy. In a pilot project of the global analysis of P. tetraurelia gene expression during autogamy, DNA microarrays were used. Statistical data analysis allowed the identification of four clusters of co-ex- pressed genes. Screening for silencing phenotypes of 15 autogamy specific genes revealed that 4 genes were essential during vegetative growth and 3 others were essential for successful sexual process.},
 type={Text},
 title={Sequencing of the largest chromosome of Paramecium tetraurelia},
 URL={http://rcin.org.pl/ichb/Content/69216/PDF/POZN271_90466_biotechnologia-2010-no4-nowak.pdf},
 keywords={biotechnology, biotechnologia},
}