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Szybka-Hrynkiewicz, Przemysław ; Janeczko, Zbigniew
Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:In this study, we examined the effect of plant growth regulators (2, 4-D, 6-BAP) and steroidal hormone (17-p-estradiol) on a quantity of diosgenin in callus tissue of Polygonatum verticillatum (L.) All. The callus grown in in vitro conditions on MS medium with; 1/1, 2/2, 5/5, 10/10 (lmg/dm31 2,4-D/lmg/dm3] 6-BAP) and 1/1/1,2/2/2, 5/5/5, 10/10/10 ([mg/dm31 2,4-D/(pM/dm3j 17-p-estradiol/(mg/dm3) 6-BAP) was used for the densitometric analysis to determine the presence of diosgenin. The content of diosgenin ranged from 0,0642 to 1,1458%. An increase in diosgenin content due to addition of 6-BAP and 2,4-D was observed. The developed procedure was estimated by statistical analysis which indicates that it can be used for routine analysis.
Relation:Biotechnologia, vol.65, 2 (2004)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
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