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Śliwińska, Elwira ; Zimny, Jarosław ; Drozdowska, Lucyna
Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:In plant tissue cultures, somaclonal variation is often observed. It can be aneffect of the changes in the individual chromosome number or in the ploidylevel. Flow cytometry, a fast and accurate method for the estimation of the nuclear DNA content, can be applied to study these changes. The DNA content indifferentiated tissues of Nicotiana tabacum cultured in vitro was estimated usingPartec CCA flow cytometer, starting from explant, through callus, up to regenerated shoots. The explant constituted stem segments of N. tabacum plants,non-transformed and transformed with gfp gene. Flow cytometric analysisshowed differences in the proportion of 2C, 4C, 8C and 16C cells in plant tissuein different culture stages. Among the regenerated plantlets originated fromnon-transformed and transformed plants, diploid, tetraploid and mixoploidforms were observed. The transformation did not influence the share of cellsrepresenting different ploidy levels in the investigated plant material.
Relation:Biotechnologia, vol.62, 3 (2003)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
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