Effect of 6-benzylaminopurine, cold-hardening and time of transferof cultures to the storage room on long-term storage of raspberry‘Mailing Seedling’ shoots in vitro

Lisek, Anna; Orlikowska, Teresa

doi:0860-7796

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Wpływ 6-benzyloaminopuryny, hartowania chłodem oraz terminu przenoszenia kultur do przechowalni na długoterminowe przechowywanie pędów maliny „Mailing Seedling”w warunkach in vitro Lisek, AnnaOrlikowska, Teresa

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Wpływ 6-benzyloaminopuryny, hartowania chłodem oraz terminu przenoszenia kultur do przechowalni na długoterminowe przechowywanie pędów maliny „Mailing Seedling”w warunkach in vitro

Effect of 6-benzylaminopurine, cold-hardening and time of transferof cultures to the storage room on long-term storage of raspberry‘Mailing Seedling’ shoots in vitro

Lisek, Anna ; Orlikowska, Teresa

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

2001

biotechnologia

Shoots of raspberry ‘Mallig Seedling’ produced on multiplication mediumwere stored /n vitro at 4°C in the dark on a medium with 0,4 mg/1 or without6-benzylaminopurine (BAP), for 18 and 24 months. The cultures were transferred to the storage room immediately after the subculture or after one-weekincubation in the growth chamber. A part of the shoots was cold-hardened forone week. Almost all the shoots survived 24 months of storage. The shootsstored on the medium containing BAP formed shoots during storage. Shootmultiplication was poor during the first passage, especially in the result of 24months storage. Time of transfer and hardening did not influence multiplicationcapacity instantly.

Biotechnologia, vol.54, 3 (2001)-.

0860-7796 ; IChB B-50

Biblioteka Instytutu Chemii Bioorganicznej PAN

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eng

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