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Możliwości wykorzystania różnych fragmentów zarodka kukurydzy (Zea mays L.) w hodowli in vitro
Subtitle: Creator:Szechyńska, Magdalena ; Filek, Maria
Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:The aim ofthe performed investigations was to determine which part oftheembryo has the optimal properties for callus induction at low concentrations of2,4-D (2 mg dm-3).The explants were cut along or across the embryo axis into 2, 3, 4 fragments and cultured on the Murashige and Skoog medium for 6 weeks. It wasfound that callus derived from mature embryos (dry seeds) is characterised bycomparable fresh and dry mass and by similar changes in size of the cells withfragments derived from mature embryos (but before the dormant) and from immature embryos. Essential difference was observed in the germination ability ofthe explants. The percentage of germination of mature embryo fragments wasthe lowest. It was demonstrated that despite of the embryo type, fragmentsoriginating from its central part appeared the most suitable for in vitro culture.In the case of these fragments the highest increase of the fresh mass of callustissue, optimal morphological features and the appearance of meristematic centres were observed.
Relation:Biotechnologia, vol.53, 2 (2001)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
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