Metadata language
Ekspresja genów Erwinia chrysanthemi kodujących pektynazy w zainfekowanej tkance roślinnej
Subtitle:Expression of Erwinia chrysanthemi genes coding pectate lyases ininfected potato tissue
Creator:Jafra, Sylwia ; Łojkowska, Ewa
Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:Erwinia chrysanthemi mutants, containing transcriptional fusion of one ofthe pectate lyase genes (pelA, pelB, peIC, pelD, pelE, pell, pelL, pelZ) with the reporter gene encoding p-giucuronidase activity, were studied for their ability to cause disease symptoms and to synthesise pectinases after inoculation of potatotubers. The strains affected in pell and pelL genes displayed a reduced virulenceon potato tubers demonstrating the important role of these isoenzymes in softroot disease. Analysis of the bacterial population showed an active multiplication of bacteria during the infection. Similar kinetics of growth were observedfor all mutants and for the wild type strain. Comparison of the mutants ?.nd thewild type strain showed that the pell, pelL and pelZ mutants synthesised reducedlevels of Pels. The expression ofpelA, pelE and pelZ is 5-fold higher in planta thanin in vitro. In contrast, both pell and pelL are highly (ł0-fold factor) induced inplanta, which is characteristic of the plant-inducible pectate lyases
Relation:Biotechnologia, vol.50, 3 (2000)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Projects co-financed by: Access: