Inny tytuł:

Expression of Erwinia chrysanthemi genes coding pectate lyases ininfected potato tissue

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstrakt:

Erwinia chrysanthemi mutants, containing transcriptional fusion of one ofthe pectate lyase genes (pelA, pelB, peIC, pelD, pelE, pell, pelL, pelZ) with the reporter gene encoding p-giucuronidase activity, were studied for their ability to cause disease symptoms and to synthesise pectinases after inoculation of potatotubers. The strains affected in pell and pelL genes displayed a reduced virulenceon potato tubers demonstrating the important role of these isoenzymes in softroot disease. Analysis of the bacterial population showed an active multiplication of bacteria during the infection. Similar kinetics of growth were observedfor all mutants and for the wild type strain. Comparison of the mutants ?.nd thewild type strain showed that the pell, pelL and pelZ mutants synthesised reducedlevels of Pels. The expression ofpelA, pelE and pelZ is 5-fold higher in planta thanin in vitro. In contrast, both pell and pelL are highly (ł0-fold factor) induced inplanta, which is characteristic of the plant-inducible pectate lyases

Czasopismo/Seria/cykl:

Biotechnologia, vol.50, 3 (2000)-.

Tom:

50

Zeszyt:

3

Strona pocz.:

202

Strona końc.:

214

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:141418 ; IChB B-46

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open