Metadata language
Starzycki, Michał ; Starzycka, Eligia ; Matuszczak, Marcin
Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:The effects of one strain Agrobacterium tumefaciens LBA 4404/pBl 121, conditions of cocultivation and growth regulators on regeneration were studied and trials of transformation wereperformed on winter oilseed rape Brassica napus L. cv. Lirajet, Valesca and Bor. Strong shootsregeneration was observed after co-cultivation without acetosyringone. Among antibiotics thatwere used in the experiments, kanamycin A and geneticin hampered whereas carbenicillin andrifampicin stimulated shoots regeneration. The presence of npt 11 gene was confirmed by PCRand Kl, K2 primers which generate a fragment of 732 bp in length.
Relation:Biotechnologia, vol.44, 1 (1999)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Projects co-financed by: Access: