Nowak, Elżbieta ; Rojek, Żaneta ; Kucharska, Danuta ; Orlikowska, Teresa
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
An efficient method for genetic Agrobacterium tumejaciens-mediated transformation of fivecultivars Gerbera hybrida was established. The youngest leaves and shoot tips from proliferatingin vitro cultures were co-cultivated with disarmed strain LBA 4404/pBI121 carrying the chimaericgenes nptll and gus. The inoculated explants were repeatedly cultured on regeneration mediumwith kanamycin and Biotaksym. After 9-10 months from 0.04 to 0.25 independently transformedshoots from one inoculated leaf and from 0.06 to 0.65 from one inoculated shoot tip wereobtained, depending on cultivar and additional treatment. Transformed shoots accounted for 5.0to 50.0% of all regenerated shoots. Additional wounding of leaves before inoculation by squeezingand scratching off increased transformation efficiency. Better results for recalcitrant to regeneration cultivars were obtained if squeezed shoot tips were inoculated. Regenerating shoots wereselected on kanamycin, screened for gas expression with X-gluc and tested by PCR for nptlland gus genes. All together 162 transgenic shoots were obtained in cvs Amber, Boy, Ferrari,Mariola and Tamara, but only 24 relatively stable lines were established.
Biotechnologia, vol.39, 4 (1997)-.
0860-7796 ; oai:rcin.org.pl:145319 ; IChB B-35
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Nov 18, 2020
Oct 30, 2020
96
https://rcin.org.pl/ichb/publication/180692
Edition name | Date |
---|---|
Wpływ eksplantatu inicjalnego na efektywność transformacji Gerbera hybrida za pomocą Agrobacterium tumefaciens | Nov 18, 2020 |
Orlikowska, Teresa Wach, Iwona Kucharska, Danuta
Kucharska, Danuta Wiśniewska-Grzeszkiewicz, Helena Orlikowska, Teresa
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Orlikowska, Teresa Jerzy, Marek
Wiejacha, Katarzyna Orlikowska, Teresa