Title:

Anti-HIV Activities and Mechanisms of Antisense Oligonucleotides

Subtitle:

Anti-HIV Activities and Mechanisms of Antisense Oligonucleotides

Creator:

Hatta, Toshifumi ; Inagawa, Takabumi ; Kuwasaki, Tomoyuki ; Kinzuka, Yasuhiro ; Takai, Kazuyuki ; Yokoyama, Shigeyuki ; Nakashima, Hideki ; Yamamoto, Naoki ; Takaku, Hiroshi

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Date issued/created:

1996

Description:

Zeszyt specjalny. Polish - Japan Issue

Subject and Keywords:

biotechnologia

Abstract:

We demonstrated that unmodified and modified (phosphorothioate) oligonucleotides preventcDNA synthesis by the AMV, MMLV, and HIV reverse transcriptases. Antisense oligonucleotide/RNA hybrids specifically arrest primer extension. The blockage involves the degradation ofthe RNA fragment bound to the antisense oligonucleotide by the reverse transcriptase associatedRNase H activity. However, the phosphorothioate oligomer inhibited polymerization by bindingto the AMV and MMLV RTs, rather than to the template RNA, whereas there was no competitivebinding of the phosphorothioate oligomer on the HIV RT during reverse transcription. Observation{of FlTC-S-ODN-reu-treated MOLT-4 cells with a confocal laser scanning microscope, revealedi diffuse fluorescence, apparently within the cytoplasm. Interestingly, fluorescent signals were; accumulated in the nuclear region of chronically infected MOLT-4/H1V-1 after a 60 min incubation. We also describe the long-term treatment of human immunodeficiency virus-infectedcells with antisense phosphorothioate oligonucleotides.

Relation:

Biotechnologia, vol.35, 4 (1996)-.

Volume:

35

Issue:

4

Start page:

116

End page:

131

Resource type:

Tekst

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-31

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

eng

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

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