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Short-chain oligosaccharide synthesis by endo-B-1,3 glucanase from Oerskovia xanthineolytica
Subtitle:Short-chain oligosaccharide synthesis by endo-B-1,3 glucanase from Oerskovia xanthineolytica
Creator:Bielecki, Stanisław ; Buchowiecka, Alicja
Publisher:Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
Date issued/created: Subject and Keywords: Abstract:An approach for enzymatic synthesis of oligosaccharides via transglycosylation with endo-p1,3-glucanase from Oerskovia xanthineolytica is described. Linear -p-l,3-glucan (laminarin) wasused as the donor of glycosyl residues, whereas nitrophenyl glycosides of different monosaccharides served as acceptors. The synthesis was performed in water-organic solvent environmentwith several combinations of donor/acceptor. Employing p-nitrophenyl-P-D-xylopyranoside as anacceptor in the presence of 30% acetonitrile resulted in the production of six new glycosidesshown by FAB MS to be di-, tri- and tetrasaccharides. This enzyme is therefore suitable for thesynthesis of short-chain oligosaccharides.
Relation:Biotechnologia, vol.33, 2 (1996)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Biblioteka Instytutu Chemii Bioorganicznej PAN
Language: Language of abstract: Temporal coverage: Rights:Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Terms of use: Digitizing institution:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Original in:Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
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