Górecka, Krystyna ; Krzyżanowska, Dorota ; Kiszczak, Waldemar ; Kowalska, Urszula ; Górecki, Ryszard
Obtaining homozygous carrot plants with the aid of androgenesis in anther cultures
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Experiments with anther cultures of 22 carrot cultivars were carried out to study the effect of various factors on the effectiveness of embryogenesis in these cultures. The factors included: the stage of microsporogenesis, genotype, training of donor plants and their growth conditions. A modified B5 medium (Gamborg, et al. 1968) containing 500 mg L’ glutamine, 100 mg L ’ serine, 0.1 mg L ' of 2,4D, 0.1 mg L ’ NAA, 100 g L ’ sucrose and 6.5 g L ’ agar were used to induce androgenesis. Regeneration was carried out on MS media and B5 with reduced concentration of sucrose at 20 g L ’ without aminoacids and hormones or with small amount of hormones. Substrates that were a mixture of various components, such as peat, sand, mineral wool and charcoal, were used for adaptation. Ploidy of the obtained plants was determined by cytometry method. Homozygosity of the plants was established using two isoenzymatic systems: PGl — phosphoglucose isomerase, and /\AT — aspartate aminotransferase. Anatomical studies of embryogenesis during antlier cultures were also carried out to confirm the androgenetic origin of embryos.
Biotechnologia, vol.81, 2 (2008)-.
0860-7796 ; oai:rcin.org.pl:73911 ; IChB B-76
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Jun 27, 2019
257
https://rcin.org.pl/ichb/publication/96733
Edition name | Date |
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Wykorzystanie androgenezy w kulturach pylnikowych do otrzymywania homozygotycznych roślin marchwi | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan