Wiater, Adrian ; Pleszczyńska, Małgorzata ; Szczodrak, Janusz ; Próchniak, Katarzyna
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Five different methods described in the literature were used for the isolation of a-(l->3)-glucans from the cell wall of fruiting bodies of Laetiporus sulphureus (Bull.iFr.) Murrill, and their comparative analysis was performed. The separated fungal biopolymers were well-characterized in respect of their structure and some physicochemical properties. Structural analyses, i.e., Fou- rier-transform infra-red (FT-IR) spectroscopy, 'FI nuclear magnetic resonance (NMR) spectroscopy and specific rotation, revealed that the alkali-soluble wall fraction from this basidiomycetous fungus contained about 56% of (1—>3)-linked a-glucans. Four out of five a-(l—>3)-glucans isolated by different methods from the mycelium of the polypore fungus L. sulphureus induced higher activity of fungal and bacterial mutanase than those obtained on mutan. Therefore, the a-(l—>3)-glucans from fruiting bodies ofL sulphureus can be used as a new alternative to streptococcal mutan, which so far has been known as the best inducer of mutanase production.
Biotechnologia, vol.81, 2 (2008)-.
0860-7796 ; oai:rcin.org.pl:73915 ; IChB B-76
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Jun 27, 2019
392
https://rcin.org.pl/ichb/publication/96748
Wiater, Adrian Pleszczyńska, Małgorzata Szczodrak, Janusz
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