Search for: [Abstract = "The accuracy of real\-time PCR \(RT\-PCR\) experiment is strongly dependent on the mathematical models of data analysis, on which the quantitative methods are based. In this review, we discuss the key steps of analysing data from real\-time PCR experiments. These are the treshold cycle determination, estimation of real\-time PCR amplification efficiency and amplicon quantification. The fit point method and the second derivative method are commonly used to determine a treshold cycle value, which is a cycle number in the early exponential phase of PCR that is used to calculate the initial amount of template DNA."]

Number of results: 1