Bilecki, Wiktor ; Okruszek, Andrzej ; Kwinkowski, Marek ; Sanak, Marek ; Przewłocki, Ryszard
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Several oligodeoxyribonucleotides in a nuclease-resistant phosphorothioate form (S-ODNs)targeted to iNOS mRNA were tested in RAW 264.7 cells as potential antisense inhibitors.Antisense S-ODNs inhibited iNOS activity in a time- and dose-dependent manner. Maximalinhibitory effect was >90% while control S-ODNs were ineffective. Application of Lipofectin Reagent, which itself had no significant effect, greatly increased antisense activity. Decreased levelsof the target mRNA, as demonstrated by reverse transcriptase-polymerase chain reaction (RTPCR), suggest the RNase H mediated mechanism.
Biotechnologia, vol.35, 4 (1996)-.
0860-7796 ; oai:rcin.org.pl:146566 ; IChB B-31
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Nov 9, 2020
Nov 9, 2020
49
https://rcin.org.pl/ichb/publication/182246
Edition name | Date |
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Antisense Oligonucleotides Against Inducible Nitric Oxide Synthase Reduce NO Production in RAW 264.7 Cells | Nov 9, 2020 |
Sławińska, Anna Siwek, Maria
Tyczewska, Agata Bąkowska- Żywicka, Kamila
Pieczyński, Marcin Bielewicz, Dawid Dolata, Jakub Szweykowska-Kulińska, Zofia
Broniarczyk, Justyna Koczorowska, Maria Magdalena Durzyńska, Julia Warowicka, Alicja Goździcka-Józefiak, Anna