- About project
- Collections
- Indexes
- Recently viewed
-
RCIN Repositories
-
INSTYTUT ARCHEOLOGII I ETNOLOGII POLSKIEJ AKADEMII NAUK
-
INSTYTUT BADAŃ LITERACKICH POLSKIEJ AKADEMII NAUK
-
INSTYTUT BADAWCZY LEŚNICTWA
-
INSTYTUT BIOLOGII DOŚWIADCZALNEJ IM. MARCELEGO NENCKIEGO POLSKIEJ AKADEMII NAUK
-
INSTYTUT BIOLOGII SSAKÓW POLSKIEJ AKADEMII NAUK
-
INSTYTUT CHEMII FIZYCZNEJ PAN
-
INSTYTUT CHEMII ORGANICZNEJ PAN
-
INSTYTUT FILOZOFII I SOCJOLOGII PAN
-
INSTYTUT GEOGRAFII I PRZESTRZENNEGO ZAGOSPODAROWANIA PAN
-
INSTYTUT HISTORII im. TADEUSZA MANTEUFFLA POLSKIEJ AKADEMII NAUK
-
INSTYTUT JĘZYKA POLSKIEGO POLSKIEJ AKADEMII NAUK
-
INSTYTUT MATEMATYCZNY PAN
-
INSTYTUT MEDYCYNY DOŚWIADCZALNEJ I KLINICZNEJ IM.MIROSŁAWA MOSSAKOWSKIEGO POLSKIEJ AKADEMII NAUK
-
INSTYTUT PODSTAWOWYCH PROBLEMÓW TECHNIKI PAN
-
INSTYTUT SLAWISTYKI PAN
-
SIEĆ BADAWCZA ŁUKASIEWICZ - INSTYTUT TECHNOLOGII MATERIAŁÓW ELEKTRONICZNYCH
-
MUZEUM I INSTYTUT ZOOLOGII POLSKIEJ AKADEMII NAUK
-
INSTYTUT BADAŃ SYSTEMOWYCH PAN
-
INSTYTUT BOTANIKI IM. WŁADYSŁAWA SZAFERA POLSKIEJ AKADEMII NAUK
-
- Adenylate cyclase activity, stimulated by dopamine, norepinephrine and forscolin, wasdemonstrated histochemically in electron microscopy of the CAj sector of Ammon’s horn inMongolian gerbil. Cobalt and stront were used as precipitating agents. The reaction productlocated in the synapses was mostly associated with the postsynaptic membranes. It was alsopresent in the plasma membrane of some nerve fibers. There were no differences in the enzymeactivity in particular layers of Ammon’s horn as well as between symmetric and asymmetric typesof synapses. The intensity of the reaction product depended mostly on the stimulator used.
- bibliogr..
- Bibliogr. 89 poz.
- Bibliogr. s. 90-100
- bibliograf..
- biblliograf..
- Brain cortex
- Electron microscopic immunocytochemical evaluation of localization and distribution of endothelins 1, 2 and 3 in the CAI hippocampal sector in rats submitted to 10 min global cerebral ischemia was performed. The studies were done in different postischemic periods (10 min, 3, 6, 12 and 24 h) with postembedding immuno-gold technique for electron microscopy. Endothelin-like immunoreactivity was found in endothelial cells of hippocampal microvessels and in astrocytes, microglia, macrophages and in some axonal endings. The most pronounced changes appeared 24 h after ischemia. At that period all structural elements of blood-brain interphase: endothelium, basal membrane and perivascular astrocytic processes showed intensive endothelin-like immunoreactivity. Especially marked immunoreactivity was found in macrophages appearing in the proximity of microvessels. It has been stressed that ischemia-induced increased content of endothelin may play an important role in the pathogenesis of postischemic tissue abnormalities.
- Organotypic cultures of hippocampus and cerebellum, established from brains of 1-3 days old rats, were exposed at different stages of development (3, 14 and 21 DIV) to 2-chloro-2’-deoxyadenosine (cladribine, 2-CdA) and 2-bromo-2’-deoxyadenosine (2-BdA) at concentrations up to 10 mikroM, for up to 10 days. Normal pattern and dynamics of differentiation and maturation of both neurons and glial cells was found with the use of light and electron microscopy.No ultrastructural abnormalities were induced by the substances tested. We conclude that 2-CdA and its sister compound 2-BdA do not exert cytotoxic effects toward normal rat central nervous system tissues in organotypic culture.
