Institutional creator:

Instytut Medycyny Doświadczalnej i Klinicznej im. M. Mossakowskiego PAN

Contributor:

Durlik, Marek (Promotor)

Description:

Bibliografia zawiera 87 pozycji ; 60 s.: il., wykr., tabl.; 30 cm. + 3 publikacje

Degree name:

doktor

Level of degree:

2

Degree discipline :

medical sciences

Degree grantor:

Instytut Medycyny Doświadczalnej i Klinicznej im. M. Mossakowskiego PAN

Abstract:

Most pancreatic cancer cases are pancreatic adenocarcinomas with poor prognosis and a low median survival. The doctoral dissertation is a series of three publications that combine the issue of prognostic factors in the population of patients with pancreatic adenocarcinoma. In the original studies, the prognostic value of factors not previously studied in the tissue of pancreatic adenocarcinomas, such as relative mtDNA copy number and DNA integrity index, were investigated. Both parameters were calculated from the data obtained by the quantitative PCR-based assay. The tumors samples originated from the patients with pathologically confirmed pancreatic ductal adenocarcinoma and the control adjacent pancreatic tissue specimens were received from surgical margins. We provided the first evidence that mitochondrial DNA (mtDNA) copy number is significantly lower in pancreatic cancer tissue (P < 0.001) compared to adjacent normal pancreatic tissue. Next, we demonstrated a significant decrease in median mtDNA copy number across the differentiation. Furthermore we showed that specimens from the tumors pathologically marked as R1 (microscopic residual tumor) had a significantly higher LINE1 300/79 ratio values than specimens from adjacent normal pancreatic tissue (P<0.05) and LINE1 300/79 ratio is a good parameter to distinguish between R0 and R1 tumors. The survival analyses in both studies failed to show a significant prognostic value for neither mtDNA copy number nor DNA integrity index. The last paper of the cycle is a review and concerns the prognostic value of cytometric parameters such as ploidy and DNA index within the population of patients with resectable pancreatic adenocarcinoma. The results of the presented studies indicate that the DNA index determined with the use of static image cytometry is a strong prognostic factor in the population of patients with pancreatic adenocarcinoma. However, the presented studies on the DNA index determined with the use of flow cytometry provided contradictory results. Most of these studies were retrospective and based on archival paraffin-embended samples. Therefore they delivered only limited evidence.

Detailed Resource Type:

PhD Dissertations

Resource Identifier:

oai:rcin.org.pl:235815

Source:

IMDiK PAN, sygn. ZS411 ; click here to follow the link

Language:

pol

Language of abstract:

eng

Rights:

Creative Commons Attribution BY 4.0 license

Terms of use:

Copyright-protected material. [CC BY 4.0] May be used within the scope specified in Creative Commons Attribution BY 4.0 license, full text available at: ; -

Digitizing institution:

Mossakowski Medical Research Institute PAS

Original in:

Library of the Mossakowski Medical Research Institute PAS

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open