Contributor:

Sarnowska, Anna (Supervisor)

Publisher:

Mossakowski Medical Research Institute Polish Academy of Sciences

Place of publishing:

Warsaw

Degree name:

doctor

Level of degree:

2

Degree discipline :

medical sciences

Degree grantor:

Mossakowski Medical Research Institute Polish Academy of Sciences

Abstract:

A major challenge in cell therapy is obtaining cells with high therapeutic potential that can survive transplantation, repopulate the tissue, and maintain long-term secretory activity. This is particularly difficult in central nervous system (CNS) regeneration, where somatic stem cells show limited neural differentiation. Evidence indicates that neural stem/progenitor cells (NSCs) are the most promising tool, as they can generate mature neural cells and secrete factors that support endogenous neurogenesis. However, their survival and proliferation after transplantation remain insufficient, and results are inconsistent. Effective NSC-based therapy requires understanding the brain microenvironment, which regulates NSC fate through biochemical, biophysical, and cellular cues. Cerebrospinal fluid (CSF) is an important component that influences NSC survival, proliferation, and differentiation, although its role in adult neurogenesis is still unclear. This study involved the isolation and culture of human, mouse, and rat NSCs and evaluating the effects of such factors as basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), glutamine, and CSF. NSCs were investigated in vitro, ex vivo, and in vivo, in a focal brain injury model. Results showed that literature discrepancies stem from species-specific differences and culture variability. Moreover, immediate post-isolation culture supports NSC viability, optimal bFGF and EGF concentrations are 20 ng/mL, the presence of glutamine and proper dissociation are essential for NSC culture. NSC migratory potential changes over time, affecting in vivo interpretation. CSF-treated NSCs showed enhanced neuroprotection and altered secretion after interaction with damaged tissue. Overall, NSCs show significant therapeutic potential for CNS disorders, but success depends on reproducing a supportive microenvironment. CSF may enhance NSC survival and function and represents a promising target for future therapeutic strategies.

Detailed Resource Type:

PhD Dissertations

Resource Identifier:

oai:rcin.org.pl:247321

Source:

IMDiK PAN, call. ZS443 ; click here to follow the link

Language:

eng

Rights:

Creative Commons Attribution BY 4.0 license

Terms of use:

Copyright-protected material. [CC BY 4.0] May be used within the scope specified in Creative Commons Attribution BY 4.0 license, full text available at: ; -

Digitizing institution:

Mossakowski Medical Research Institute PAS

Original in:

Library of the Mossakowski Medical Research Institute PAS

Access:

Open