Mitochondrial damage following exposure of organotypic cultures of human malignant gliomas to 2-chloro- and 2-bromo-2’-deoxyadenosine

Matyja, Ewa; Grieb, Paweł; Ryba, Mirosław; Jagielski, Janusz; Mossakowski, Mirosław Jan (1929–2001)

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Mitochondrial damage following exposure of organotypic cultures of human malignant gliomas to 2-chloro- and 2-bromo-2’-deoxyadenosine Matyja, EwaGrieb, PawełRyba, MirosławJagielski, JanuszMossakowski, Mirosław Jan (1929–2001)

Mitochondrial damage following exposure of organotypic cultures of human malignant gliomas to 2-chloro- and 2-bromo-2’-deoxyadenosine

Zmiany w mitochondriach po ekspozycji organotypowych hodowli ludzkich złośliwych guzów glejopochodnych na 2-chloro i 2-bromo-2’-dezoksyadenozynę

Matyja, Ewa ; Grieb, Paweł ; Ryba, Mirosław ; Jagielski, Janusz ; Mossakowski, Mirosław Jan (1929–2001)

Medical Publishing House

Warsaw

1996

glial tumors ; cladribine (2-CdA) ; 2-bromo-2’-deoxyadenosine ; organotypic cultures ; mitochondrial toxicity

The effects of 2-chloro-2’-deoxyadenosine (cladribine, 2-CdA) and a closely related compound 2-bromo-2’-deoxyadenosi- ne (2-BdA) on organotypic cultures of human malignant gliomas were studied with the use of electron microscopy. The cultures grown from surgical biopsies included six glioblastomas, three anaplastic astrocytomas and low-grade fibrillary astrocytoma. After 6 to 10 days of the in vitro growth the cultures were exposed to 0.3-10 /iM 2-CdA or 2-BdA for 1 to 10 days. Mitochondrial swelling and disappearance of cristae following exposure to the tested substances were observed, but only in highly anaplastic (low-differentiated) tumor cells. The mitochondrial toxicity was dose- and time-dependent, and no difference was found between the effect of 2-CdA and 2-BdA.

Folia Neuropathologica