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Object

Title: 2-chloro- and 2- bromo-2’ deoxy adenosine have no effect on the morphology of rat neuronal and glial cells in organotypic culture

Subtitle:

Brak wpływu 2-chloro i 2-bromo-2’-dezoksyadenozyny na morfologię komórek nerwowych i glejowych szczura w hodowli organotypowej

Publisher:

Medical Publishing House

Place of publishing:

Warsaw

Type of object:

Journal/Article

Abstract:

Organotypowe hodowle hipokampa i móżdżku, założone z materiału pobranego od szczurów w 1-3 dni po urodzeniu, hodowano przez 3, 10 i 14 dni. Następnie hodowle te, w różnych stadiach rozwoju, eksponowano na 2-chloro-2’-dezoksyadenozynę (cladribine,2-CdA) i 2-bromo-2’-dezoksyadenozynę (2-BdA) w stężeniachdo 10 mikroM przez okres do 10 dni. Metodami mikroskopiiświetlnej i elektronowej stwierdzono normalny wzorzec i dynamikęróżnicowania i dojrzewania komórek nerwowych i glejowych. Badane substancje nie powodowały zaburzeń ultrastruktury komórek.2-CdA i siostrzana substancja nie wykazują efektów cytotoksycznych wobec normalnych tkanek ośrodkowego układu nerwowego szczura.

References:

Organotypic cultures of hippocampus and cerebellum, established from brains of 1-3 days old rats, were exposed at different stages of development (3, 14 and 21 DIV) to 2-chloro-2’-deoxyadenosine (cladribine, 2-CdA) and 2-bromo-2’-deoxyadenosine (2-BdA) at concentrations up to 10 mikroM, for up to 10 days. Normal pattern and dynamics of differentiation and maturation of both neurons and glial cells was found with the use of light and electron microscopy.No ultrastructural abnormalities were induced by the substances tested. We conclude that 2-CdA and its sister compound 2-BdA do not exert cytotoxic effects toward normal rat central nervous system tissues in organotypic culture.

Relation:

Folia Neuropathologica

Volume:

34

Issue:

1

Start page:

41

End page:

47

Detailed Resource Type:

Article

Format:

pdf

Resource Identifier:

oai:rcin.org.pl:71893

Language:

eng

Language of abstract:

eng ; pol

Rights:

Creative Commons Attribution BY 4.0 license

Terms of use:

Copyright-protected material. [CC BY 4.0] May be used within the scope specified in Creative Commons Attribution BY 4.0 license, full text available at: ; -

Digitizing institution:

Mossakowski Medical Research Institute PAS

Original in:

Library of the Mossakowski Medical Research Institute PAS

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open

Object collections:

Last modified:

Feb 1, 2022

In our library since:

May 30, 2019

Number of object content downloads / hits:

215

All available object's versions:

https://rcin.org.pl/imdik/publication/94267

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